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cell sorting architecture platform size viva

Platform Size

Reducing the size of traditionally large cell sorter system has been an industry challenge. Engineering support of charged droplet sorting technology has constrained efforts to date, carrying with it complexity in instrument installation and use.

Approach

The Cytonome Viva™ system finally breaks through these barriers, with the world’s smallest design incorporating standard charged droplet technology. All engineering support is now on-board the industry’s smallest platform, including all optics, fluidics, pressure sources, and computer interface.

Benefits

  • Ease-of-use sorting for virtually all lab personnel
  • Plug-in system installation, period
  • Efficient use of laboratory space
  • Biohazard containment; fits in std. biohazard hood
  • Extremely accessible price point for most laboratory budgets
cell sorting architectures sort speed scaling hydris

Sort Speed Scaling

Virtually all current high-performance cell sorters utilize charged droplet sorting technology.

Attempts at increasing sorting efficiency and speed have largely focused on higher stream pressures, higher cell concentrations and faster computer analysis. Yet the physics associated with single stream charged droplet sorting places theoretical limits on sorting performance, and today’s current single stream systems have effectively reached these limitations and their concomitant effects on cell integrity. Advancing far beyond them to address several of today’s industry needs is not practical with single stream designs.

Approach

Cytonome Hydris™ system sorting architecture bypasses these theoretical physical barriers and enables researchers to dramatically scale-up their sum total sorting speeds and thus overall cell purification yields. Using a proprietary multi-headed sorting design, traditional charged droplet sorting can now be scaled across a wide performance range as dictated by the user. Each sorting head can be operated and controlled as an independent system, or linked together as needs dictate.

Benefits

  • Scalable, high-throughput combined sort speeds
  • Sorting efficiency and reliability
  • Proven performance, reliability in multi-head design
  • Flexibility in sorting applications
cell sorting architecture sort speed parallelism chip

Sort Speed Parallelism

Microfluidic cell sorting permits a new way to address higher sorting speeds.

The efficient yet gentle cell sorting of individual, sealed microchannels, when combined in great numbers, provides sum-total overall sorting speeds once only imagined, and in a safe and sterile manner. Each microchannel functions as a single ‘cell sorter’; when the performance of 24, 48 or 72 of these channels is combined, extremely fast sum total sorting speeds can be achieved without higher pressures, cell damage or aerosol creation.

Approach

Cytonome GigaSort™ system sorting architecture is unique and without peer in the industry. Using a proprietary, multi-channel microfluidics design, 24 or more individual microsorting channels are arrayed within single, sealed glass chip. Using a technology known as PicoPulseTM, cells of interest are gently deflected into collection channels by means of a fluid pulse, without the high pressures, shearing forces or sheath fluid limtations imposed by charged droplet nozzle design. Cells can be sorted in a variety of compatible fluids, and no aerosols are created during sorting.

Benefits

  • Increased sort speeds by design
  • Increased cell integrity, survival
  • Sorting efficiency and speed
  • Sterility of sorting
  • Flexibility in sorting applications
  • Safety for lab personnel