cell sorting architecture platform size viva

Platform Size

Failed attempts to reduce the size of traditionally large cell sorter systems have constrained efforts to date, carrying with it complexity in instrument installation and use.

Approach

The Cytonome Viva™ system finally breaks the size barrier with the world’s smallest design incorporating standard charged droplet technology. All engineering support is now on-board the industry’s smallest platform, including all optics, fluidics, pressure sources, and computer interface.

Benefits

  • Ease-of-use sorting for virtually all lab personnel
  • Plug-in system installation, period
  • Efficient use of laboratory space
  • Biohazard containment; fits in std. biohazard hood
  • Extremely accessible price point for most laboratory budgets
cell sorting architectures sort speed scaling hydris

Sort Speed Scaling

Virtually all current high-performance cell sorters utilize charged droplet sorting technology.

Attempts at increasing sorting efficiency and speed have largely focused on higher stream pressures, higher cell concentrations and faster computer analysis. Yet the physics associated with single stream charged droplet sorting places theoretical limits on sorting performance, and their concomitant effects on cell integrity.

Approach

Cytonome Hydris™ system's sorting architecture lessens these restrictions and enables researchers to dramatically scale-up their sorting speeds and thus, overall cell purification yields. Using a proprietary multi-headed sorting design, traditional charged droplet sorting can now be operated and controlled as an independent system, or linked together as needs dictate.

Benefits

  • Scalable, high-throughput combined sort speeds
  • Sorting efficiency and reliability
  • Proven performance, reliability in multi-head design
  • Flexibility in sorting applications
cell sorting architecture sort speed parallelism chip

Sort Speed Parallelism

Microfluidic cell sorting permits a new way to address higher sorting speeds.

The efficient yet gentle cell sorting of individual, sealed microchannels, when combined in great numbers, provides sorting speeds once only imagined. Each microchannel functions as a single ‘cell sorter’ within a single glass chip. When the performance of 24, 48 or 72 of these channels is combined, extremely fast sorting speeds can be achieved without higher pressures, cell damage or aerosol creation.

Approach

Cytonome GigaSort™ system's sorting architecture is trailblazing and without peer in the industry. Using a technology known as PicoPulseTM, cells of interest are gently deflected into collection channels by means of a fluid pulse, without the high pressures, shearing forces or sheath fluid limtations imposed by charged droplet nozzle design.

Benefits

  • Increased sort speeds by design
  • Increased cell integrity, survival
  • Sorting efficiency and speed
  • Sterility of sorting
  • Flexibility in sorting applications
  • Safety for lab personnel