Upon exiting the nozzle, pass through a laser beam in single-file, specific cell-containing droplets that have been identified as members of a target population are electrostatically charged. Charged droplets are then deflected away from the main fluid stream into collection vessels.
Hydrodynamic focusing ensures that cells pass the light excitation source in single file for accurate characterization with no exposed fluid streams, droplets, nozzles or electrostatic charges. Target cells are selected through a fast fluidic-switch mechanism. The enclosed design supports sterile sorting and delivers a gentle, aerosol free method for cell purification.
The Cytonome Viva™ system finally breaks the size barrier with the world’s smallest design incorporating standard charged droplet technology. All engineering support is now on-board the industry’s smallest platform, including all optics, fluidics, pressure sources, and computer interface.
Attempts at increasing sorting efficiency and speed have largely focused on higher stream pressures, higher cell concentrations and faster computer analysis. Yet the physics associated with single stream charged droplet sorting places theoretical limits on sorting performance, and their concomitant effects on cell integrity.
Cytonome Hydris™ system's sorting architecture lessens these restrictions and enables researchers to dramatically scale-up their sorting speeds and thus, overall cell purification yields. Using a proprietary multi-headed sorting design, traditional charged droplet sorting can now be operated and controlled as an independent system, or linked together as needs dictate.
The efficient yet gentle cell sorting of individual, sealed microchannels, when combined in great numbers, provides sorting speeds once only imagined. Each microchannel functions as a single ‘cell sorter’ within a single glass chip. When the performance of 24, 48 or 72 of these channels is combined, extremely fast sorting speeds can be achieved without higher pressures, cell damage or aerosol creation.
Cytonome GigaSort™ system's sorting architecture is trailblazing and without peer in the industry. Using a technology known as PicoPulseTM, cells of interest are gently deflected into collection channels by means of a fluid pulse, without the high pressures, shearing forces or sheath fluid limitations imposed by charged droplet nozzle design.